Basic Stereology

Stereology.

1.1 組織標本を使用する定量解析が抱える諸問題 組織標本の解析は定性的な所見から始まる.顕微鏡を用いた丁寧な観察によって初めて得られる検体の 大きさや分布,形態などについての詳細な情報は,全ての研究の基礎となるべき重要なものである.しか しながら,定性的なデータだけでは,統計学的な検討が難しく,結果が主観的なものになりやすい.この ため,生理的組織と病的組織の比較など,さらなる研究を行う際には,定量的なデータが必要になる.定 量解析の基本的な命題は,いかにして検体の正確な数を客観的に求めることができるか,ということであ る.組織標本に基づく定量解析は単純に思われるが,正確なデータを得ることは実は容易ではない. 最大の問題は,サンプリングバイアスである(図1).これは,標本抽出の際に母集団を代表していない 検体が選ばれるために生じる偏りのことであり,2次元的な切片を使って定量解析を行...

basic application of stereology in histology and medical sciences

18th European Stereology Course

An International Stereology course approved by the International Society for Stereology. This course will provide participants with a practical introduction to recent advances in stereology. There will be ample opportunity for discussion between participants and the experienced instructors on specific stereological problems related to the participants’ own projects. Particular emphasis will be ...

Quantitative microscopy of the lung: a problem-based approach. Part 1: basic principles of lung stereology.

The growing awareness of the importance of accurate morphometry in lung research has recently motivated the publication of guidelines set forth by a combined task force of the American Thoracic Society and the European Respiratory Society (20). This official ATS/ERS Research Policy Statement provides general recommendations on which stereological methods are to be used in quantitative microscop...

Stereology as inverse problem

Stereology is the part of imaging science in which the three-dimensional structure of a body is determined from two-dimensional views. Although it is relatively easy to determine volume information from 2-D slices, it is nontrivial in general to determine other physical properties such as internal surface areas unless the medium is known to have some simple symmetry such as isotropy. For this r...